Breast cancer intracranial metastases

Intracranial metastases etiology


RET expression was characterized in a cohort of patients with primary and brain metastatic tumors. RET functionality was assessed using pharmacological inhibition and gene silencing in patient-derived brain metastatic tumor explants and in vivo models, organoid models, and brain organotypic cultures. RNA sequencing was used to uncover novel brain metastatic relevant RET mechanisms of action.

Results: A statistically significant enrichment of RET in brain metastases was observed in estrogen receptor-positive breast cancer, where it played a role in promoting cancer cell adhesion, survival, and outgrowth in the brain. In vivo, RET overexpression enhanced brain metastatic competency in patient-derived models. At a mechanistic level, RET overexpression was found to enhance the activation of gene programs involved in cell adhesion, requiring EGFR cooperation to deliver a pro-brain metastatic phenotype.

The results illustrate, for the first time, the role of RET in regulating colonization and outgrowth of breast cancer intracranial metastases and provide data to support the use of RET inhibitors in the management strategy for patients with breast cancer brain metastases 3)

The status of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor type 2 (HER2) may be altered in the time window between the emergence of the primary breast tumor and the development of metastases.

CSF CEA: levels>1ng/ml are reported with a leptomeningeal spread of lung Ca (89%),breast cancer (60–67%), malignant melanoma (25–33%), and bladder Ca. May be normal even in CEA-secreting cerebral metastases if they don’t communicate with the subarachnoid space. Only carcinomatous meningitis from lung or breast cancer consistently elevates CSF CEA in the majority of patients.

Predictors of long-term survival included HER2+ status (P = .041) and treatment with TT (P = .046). A limited number of patients (11%) died of central nervous system (CNS) causes. A predictor of CNS-related death was the development of leptomeningeal disease after SRS (P = .025), whereas predictors of non-CNS death included extracranial metastases at first SRS (P = .017), triple-negative breast cancer (P = .002), a Karnofsky Performance Status of <80 at first SRS (P = .002), and active systemic disease at last follow-up (P = .001). Only 13% of patients eventually needed whole-brain radiotherapy. Among the long-term survivors, none died of CNS progression 4).


Brain metastases significantly affect morbidity and mortality rates for patients with metastatic breast cancer.

Poor penetration of systemic therapies into the CNS can create a reservoir for tumor growth in the brain despite excellent control of extracranial disease.


Delayed toxicity after whole brain radiation therapy (WBRT) is of increasing concern in patients who survive more than one year with breast cancer intracranial metastases.


1)
Duchnowska R, Dziadziuszko R, Czartoryska-Arłukowicz B, Radecka B, Szostakiewicz B, Sosińska-Mielcarek K, et al. Risk factors for brain relapse in HER2-positive metastatic breast cancer patients. Breast Cancer Res Treat. 2009;117:297–303.
2)
Tsukada Y, Fouad A, Pickren JW, Lane WW. Central nervous system metastasis from breast carcinoma. Autopsy study. Cancer. 1983;52:2349–2354.
3)
Jagust P, Powell AM, Ola M, Watson L, de Pablos-Aragoneses A, García-Gómez P, Fallon R, Bane F, Heiland M, Morris G, Cavanagh B, McGrath J, Ottaviani D, Hegarty A, Cocchiglia S, Sweeney KJ, MacNally S, Brett FM, Cryan J, Beausang A, Morris P, Valiente M, Hill ADK, Varešlija D, Young LS. RET overexpression leads to increased brain metastatic competency in luminal breast cancer. J Natl Cancer Inst. 2024 Jun 10:djae091. doi: 10.1093/jnci/djae091. Epub ahead of print. PMID: 38852945.
4)
Mashiach E, Alzate JD, De Nigris Vasconcellos F, Bernstein K, Donahue BR, Schnurman Z, Gurewitz J, Rotman LE, Adams S, Meyers M, Oratz R, Novik Y, Kwa MJ, Silverman JS, Sulman EP, Golfinos JG, Kondziolka D. Long-term Survival From Breast Cancer Brain Metastases in the Era of Modern Systemic Therapies. Neurosurgery. 2023 Aug 15. doi: 10.1227/neu.0000000000002640. Epub ahead of print. PMID: 37581437.
  • breast_cancer_intracranial_metastases.txt
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