The border zone of different tissues is often the object of discussion, considering that the definition of the border is always ambiguous, especially in cases of tumor. The population of different cells in this area largely depends on the character of those cells in terms of the ability of cell motility and the status of tissue barrier, such as extracellular matrices. Gliomas, especially malignant gliomas, are known to possess a highly invasive nature. The surgical extent of resection is determined by the intraoperative macroscopic appearance, sometimes assisted by the information of a navigation system based on the preoperative images, that is easily influenced by the intraoperative brain shift in many cases. Therefore, the surgery often results in an incomplete resection ¹⁾.