RNA sequencing analysis) is a powerful technique used to study the transcriptome—the complete set of RNA transcripts produced by the genome under specific conditions. Here's a concise definition:
RNA-seq Analysis
RNA-seq analysis refers to the bioinformatic and statistical processing of RNA sequencing data to quantify, compare, and interpret gene expression levels across samples.
It involves:
Sequencing RNA transcripts (usually after converting to cDNA)
Mapping the reads to a reference genome or transcriptome
Quantifying expression levels of genes or transcripts
Detecting differentially expressed genes (DEGs) between conditions
Exploring alternative splicing, fusion transcripts, non-coding RNAs, and allele-specific expression
This method allows researchers to:
Identify genes involved in disease
Compare expression between cell types or treatments
Discover novel transcripts
Tags: transcriptomics, gene expression, NGS, bioinformatics, differential expression