PAK1 and PAK4 may be stronger predictors of immune characteristics and are more suitable as drugs and molecular therapeutic targets. Furthermore, Cox regression analysis revealed that a PAK gene signature could be used as an independent prognostic factor for low-grade glioma (LGG) and glioblastoma (Glioblastoma). Gene set enrichment analysis (GSEA) analysis indicated that PAK genes may affect the occurrence and development of Glioblastoma through the PI3K signaling pathway. Further experiments verified that PAK1 and AKT1 have a significant interaction in Glioblastoma cells, and inhibiting the overactivation of PAK1 can significantly inhibit the proliferation of Glioblastoma cells.

The study provides a rationale for further research on the prognostic and therapeutic potential of PAKs in human tumors ¹⁾.