The interpeduncular fossa is a somewhat rhomboid-shaped area of the base of the brain, limited in front by the optic chiasma, behind by the antero-superior surface of the pons, antero-laterally by the converging optic tracts, and postero-laterally by the diverging cerebral peduncles.

The structures contained in it, from behind forward, are the posterior perforated substance, corpora mamillaria, tuber cinereum, infundibulum, and pituitary gland.

The subtemporal approach is one of the surgical routes used to reach the interpeduncular fossa.