glioma_cell_line

Glioma cell line

C6

C6 cancer cell line

GSC23

GSC23

J3T

J3T

LN229

LN229

SNB19

SNB19

T98G

T98G

U87

U87MG

U105

U105

U251

U251

U373 MG

U373 MG

mis-cultured glioma cells can acquire unnatural drug sensitivity, which would have devastating effects for clinical translations 1).

In 2006, inspired by neural stem cell (NSC) culture conditions, the Fine lab used serum-free, EGF/FGF-2-supplemented Neurobasal medium to cultivate primary glioma cells and found that these cells remained more similar to the parental tumors than those cultured in serum-containing DMEM medium 2).

Extracellular vesicles secreted by human glioma cells contain a wealth of tumor-specific proteins and nucleic acids that can be isolated from patients with these neoplasms. Thus, EV contribute to the development of biomarkers, and additionally have certain therapeutic potential for possible use in neurooncology and neurosurgery 3).

Results suggest that glioma cells themselves express Angiopoietin 2 and that expression may be induced by hypoxic stimulation and may play a crucial role in the vessel maturation, angiogenesis, and vessel regression in malignant glioma 4).

1)
Ledur PF, Onzi GR, Zong H, Lenz G. Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries? Oncotarget. 2017 Aug 11;8(40):69185-69197. doi: 10.18632/oncotarget.20193. eCollection 2017 Sep 15. Review. PubMed PMID: 28978189; PubMed Central PMCID: PMC5620329.
2)
Lee J, Kotliarova S, Kotliarov Y, Li A, Su Q, Donin NM, Pastorino S, Purow BW, Christopher N, Zhang W, Park JK, Fine HA. Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell. 2006 May;9(5):391-403. PubMed PMID: 16697959.
3)
Santiago-Dieppa DR, Gonda DD, Cheung VJ, Steinberg JA, Carter BS, Chen CC. Extracellular Vesicles as a Platform for Glioma Therapeutic Development. Prog Neurol Surg. 2018;32:172-179. doi: 10.1159/000469689. Epub 2018 Jul 10. PubMed PMID: 29990983.
4)
Koga K, Todaka T, Morioka M, Hamada J, Kai Y, Yano S, Okamura A, Takakura N, Suda T, Ushio Y. Expression of angiopoietin-2 in human glioma cells and its role for angiogenesis. Cancer Res. 2001 Aug 15;61(16):6248-54. PubMed PMID: 11507079.
  • glioma_cell_line.txt
  • Last modified: 2024/06/07 02:55
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