Antibiotic resistance

• Synergistic Antibacterial Effects of Plant Extracts and Essential Oils Against Drug-Resistant Bacteria of Clinical Interest
• Post-Surgical Central Nervous System Infections in the Era of Multidrug Antibiotic Resistance in Greece-A Single-Center Experience of a Decade
• Critical contributions of neuronal subtypes to pediatric drug-resistant focal dysplasia
• Postoperative Meningitis Caused by Multidrug-Resistant Pathogens: A Case Report
• Comparative effectiveness of monotherapy vs. combination therapy for postoperative central nervous system infections in neurosurgical patients: a retrospective cohort study
• Mycobacterium abscessus infection of uncertain etiology following lumbar spine decompression and instrumentation: illustrative case
• Molecular epidemiological analysis and research on resistance and virulence of carbapenem-resistant Klebsiella pneumoniae in a tertiary hospital from 2016 to 2023
• Healthcare-Associated Infections: The Role of Microbial and Environmental Factors in Infection Control-A Narrative Review

Surgical antibiotic prophylaxis refers to the administration of antibiotics before a surgical procedure to prevent the development of surgical site infections (SSIs). The goal of prophylactic antibiotics is to reduce the risk of bacterial contamination during surgery and to prevent postoperative infections. However, it is important to use antibiotics judiciously to minimize the development of antibiotic resistance.

There has been an upward trend in Carbapenem-resistant Klebsiella pneumoniae infections in China. This epidemiological trend needs to be examined to enable better disease control. Chen et al. sought to analyze the genomic characteristics, including the prevalent sequence type (ST), resistance, virulence, and evolutionary relationship, of K. pneumoniae strains isolated from patients with different types of infections in northern China to provide theoretical support for the effective prevention and control of the evolution and transmission of K. pneumoniae.

The STs were analyzed using multi-locus sequence typing (MLST). Drug susceptibility tests were used to examine the resistance of these STs to various antibiotics. A phylogenetic tree of these isolates was constructed using the National Center for Biotechnology Information genome as the reference. Antibiotic resistance genes were identified by comparing the genomic sequences against those in the Comprehensive Antibiotic Resistance Database. Virulence genes were identified using the Virulence Factor database, while the pathogenicity of the isolates was predicted using PathogenFinder.

In total, 38 clinical isolates of K. pneumoniae were identified and sequenced by high-throughput sequencing. Multidrug-resistant ST11 and hypervirulent ST23 were found to be the prevalent K. pneumoniae strains. The distribution of the ST11 strains was strongly correlated with stays in the neurosurgery department (chi square test, P=0.02), while the ST23 strains were more frequently isolated from patients with liver abscesses and gallbladder infections. The ST23 strains were significantly more pathogenic than the other STs (Wilcox test, P<0.001). The resistance analysis showed that the rmtB genes were significantly correlated with amikacin resistance (P<2.2e-16, R2=1). The ST11 strains were also found to co-harbor the KPC-2, rmtB, and TEM-1 genes. To the best of our knowledge, this is the first study to report on the dissemination of such multidrug-resistant K. pneumoniae ST11 strains in Tianjin.

The carbapenem-resistant K. pneumoniae (CRKP) ST11 may become highly virulent K. pneumoniae (CR-hvKP) due to the acquisition of virulence plasmids. Attention should be paid to the evolutionary pressure of a caused by the overuse of antibiotics, which may trigger the further development of multidrug-resistant K. pneumoniae infections ¹⁾.

This study provides a valuable contribution to understanding carbapenem-resistant K. pneumoniae infections, particularly the roles of ST11 and ST23 strains in northern China. Despite its limitations, the genomic analysis highlights critical issues regarding antibiotic resistance, virulence, and the potential evolution of CR-hvKP. Future research incorporating larger sample sizes, clinical data, and experimental validation will further solidify these findings and support effective prevention and control strategies.