VSV-G fusion refers to the use of the vesicular stomatitis virus glycoprotein G (VSV-G) to enhance the fusion of engineered delivery systems—such as extracellular vesicles (EVs)—with the plasma membrane of target cells.

• Envelope glycoprotein from Vesicular Stomatitis Virus
• Enables pH-dependent membrane fusion in endosomes
• Broad host cell range (non-specific tropism)
• Used to pseudotype viral and EV-based delivery vectors

VSV-G is incorporated into EV membranes to:

• Promote efficient fusion with recipient cells
• Improve cytosolic delivery of loaded therapeutic cargo (e.g., Cas9, Cre)
• Enhance in vivo delivery efficiency

• Immunogenic in clinical settings
• Lacks tissue specificity