The main difference between multispectral and hyperspectral is the number of bands and how narrow the bands are. Multispectral imagery generally refers to 3 to 10 bands. … Hyperspectral imagery consists of much narrower bands (10-20 nm). A hyperspectral image could have hundreds or thousands of bands

Hyperspectral and multispectral imaging (HMSI) in medical applications provides information about the physiology, morphology, and composition of tissues and organs. The use of these technologies enables the evaluation of biological objects and can potentially be applied as an objective assessment tool for medical professionals.

A study investigated HMSI systems for their usability in medical applications.

Approach: Four HMSI systems (one hyperspectral pushbroom camera and three multispectral snapshot cameras) were examined and a spectrometer was used as a reference system, which was initially validated with a standardized color chart. The spectral accuracy of the cameras reproducing chemical properties of different biological objects (porcine blood, physiological porcine tissue, and pathological porcine tissue) was analyzed using the Pearson correlation coefficient.

Results: All the HMSI cameras examined were able to provide the characteristic spectral properties of blood and tissues. A pushbroom camera and two snapshot systems achieve Pearson coefficients of at least 0.97 compared to the ground truth, indicating a very high positive correlation. Only one snapshot camera performs moderately to high positive correlation (0.59 to 0.85).

The knowledge of the suitability of Hyperspectral and multispectral imaging (HMSI) cameras for accurate measurement of chemical properties of biological objects offers a good opportunity for the selection of the optimal imaging tool for specific medical applications, such as organ transplantation ¹⁾.