The specimens were extracted during the first 12 h postmortem and placed in 10% formalin solution for 8 weeks. The brains were washed under running water and frozen to −15°C for 2 weeks

During the 1930s, white matter tracts began to assume relevance for neurosurgery, especially after Cajal's work. In many reviews of white matter neurobiology, the seminal contributions of Josef Klingler (1888-1963) and their neurological applications have been overlooked ¹⁾.

This freezing technique, first described by Josef Klingler and later revitalized by Uğur Türe et al. has already been reported to have a good correlation with diffusion tensor imaging (DTI) tractography ²⁾.