====== TRPA1 ======

{{rss>https://pubmed.ncbi.nlm.nih.gov/rss/search/1nwkqzEtY5u46co9__SKQdAGiAPZrG2eAxAXLpsVdiIJI2OgVZ/?limit=15&utm_campaign=pubmed-2&fc=20230107133954}}
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Astrocytic [[transient receptor potential]] ankyrin 1 (TRPA1) in glial phenotype transformation in [[neuroinflammation]] after [[intracerebral hemorrhage]] (ICH). Wild-type astrocytes and TRPA1-/- astrocytes were subjected to 6-h hemin treatment, and the [[calcium]] ions and [[transcriptome]] [[sequencing]] were assessed. A mouse autologous blood injection ICH model was established to evaluate the proliferation and [[phenotype]]s of [[astrocyte]]s and [[microglia]] around the [[hematoma]]. The [[neuroinflammation]] and behavioral performance of wild-type ICH mice and TRPA1-/- ICH mice were assessed. [[Knockout]] of astrocytic TRPA1 decreased [[calcium]] ions of [[astrocyte]]s after [[hemin]] treatment in-vitro, and microglial and astrocytes around the hematoma proliferated after the ICH model. Furthermore, RNA-sequencing (RNA-seq), immunofluorescence, and Western blotting results showed that the activated astrocytes transformed into the A2 phenotype in TRPA1-/- ICH mice. The 'ameboid' microglia were observed around the hematoma in TRPA1-/- ICH mice. The proliferation of A2 astrocytes and 'ameboid' microglia ameliorated the neuroinflammation after ICH. The [[inflammatory response]] was reduced by inhibiting the mitogen-activated protein kinase/nuclear factor kappa-B signaling pathway, and neurologic deficits were improved in TRPA1-/- ICH mice compared with wild-type ICH mice. This research suggests that astrocytic TRPA1 is a new therapeutic target to rescue [[neuroinflammation]] by modulating the glial phenotype after ICH
((Xia M, Chen YJ, Chen B, Ru X, Wang J, Lin J, Tang X, Chen W, Hu R, Li W, Feng H. Knockout of transient receptor potential ankyrin 1 (TRPA1) modulates the glial phenotype and alleviates perihematomal neuroinflammation after intracerebral hemorrhage in mice via MAPK/NF-κB signaling. Neuroreport. 2023 Feb 1;34(2):81-92. doi: 10.1097/WNR.0000000000001862. Epub 2022 Nov 30. PMID: 36608163.))