The cell culture wound-closure and the transwell migration and invasion assays are widely used in the scientific community.

These tests can provide the necessary data that may allow for an understanding of how well a particular cell type can spontaneously migrate or respond to a chemo-attractant and directionally migrate toward it. Several migratory phenotypes have been described. Cells may migrate in a single cell form such as seen in mesenchymal or amoeboid-like movement or by multicellular movement labeled collective migration or cell streaming
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Jagged1 is the ligands of the Notch signaling and has been shown to promote [[glioma stem cell]]s in [[glioblastoma]]. 

Survival data from [[R2 genomics analysis]], the [[Cancer Genome Atlas]] (TCGA), the [[Chinese Glioma Genome Atlas]] (CGGA) and visualization platform [[database]] were used to evaluate the effects of Jagged1 on overall patient [[survival]]. Hai et al., investigated Jagged1 induced the [[glioma stem cell]]s invasion by matrix degradation assays and [[Transwell cell invasion assay]]s in vitro, then they further explored the underlying molecular mechanisms using Co-immunoprecipitation (co-IP) analysis.

High expression of Jagged1 in human glioma was associated with poor survival. Clinical data analysis showed that the Jagged1 was positively correlated with [[NF-κB(]]p65). Jagged1-induced invasion of [[glioma stem cell]]s through activation of NF-κB(p65) pathway. In vivo, [[knockdown]] of Jagged1 could suppress the [[tumorigenicity]] of GICs cells through NF-κB(p65) signaling.

Insights gained from these findings suggest that Jagged1 plays an important oncogenic role in GICs malignancy by activation of NF-κB(p65) signaling, and Jagged1 could be employed as an effective therapeutic target for GICs
((Hai L, Liu P, Yu S, Yi L, Tao Z, Zhang C, Abeysekera IR, Li T, Tong L, Ma H,
Liu B, Xie Y, Zhou X, Lin Y, Zhu M, Zhang K, Ren B, Ming H, Huang Y, Yang X.
Jagged1 is Clinically Prognostic and Promotes Invasion of Glioma-Initiating Cells
by Activating NF-κB(p65) Signaling. Cell Physiol Biochem. 2018 Dec
21;51(6):2925-2937. doi: 10.1159/000496044. [Epub ahead of print] PubMed PMID:
30580328.
)).