[[RNA sequencing]] analysis) is a powerful technique used to study the [[transcriptome]]—the complete set of [[RNA]] [[transcript]]s produced by the [[genome]] under specific conditions. Here's a concise definition:

====== RNA-seq Analysis ======

RNA-seq analysis refers to the bioinformatic and statistical processing of RNA sequencing data to quantify, compare, and interpret gene expression levels across samples.

It involves:

Sequencing RNA transcripts (usually after converting to cDNA)

Mapping the reads to a reference genome or transcriptome

Quantifying expression levels of genes or transcripts

Detecting differentially expressed genes (DEGs) between conditions

Exploring alternative splicing, fusion transcripts, non-coding RNAs, and allele-specific expression

This method allows researchers to:

Identify genes involved in disease

Compare expression between cell types or treatments

Discover novel transcripts

((RNA-seq is widely used in cancer biology, developmental studies, and personalized medicine))

Tags: [[transcriptomics]], [[gene expression]], [[NGS]], [[bioinformatics]], [[differential expression]]