KNG1 (Kininogen 1) is a Protein Coding gene. Diseases associated with KNG1 include Angioedema, Hereditary, 6, and High Molecular Weight Kininogen Deficiency. Among its related pathways are "Agents Acting on the Renin-Angiotensin System Pathway, Pharmacodynamics" and GPCR downstream signaling. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and cysteine-type endopeptidase inhibitor activity. An important paralog of this gene is HRG.



Since the inhibitory effect of [[KNG1]] on [[glioma]] has been proved, this study further explores the [[regulation]] of the [[lncRNA]]/[[miRNA]] axis on KNG1 in glioma.

The miRNAs that target KNG1 and the [[lncRNA]] that target [[miR 942]]-5p were predicted by [[bioinformatics]] [[analysis]] and verified by [[experiment]]s. The correlations between miR-942-5p and the survival of patients and between KNG1 and miR-942-5p were analyzed. After [[transfection]], [[cell migration]], [[invasion]], [[proliferation]], and [[cell cycle]] were detected through [[wound healing]], [[Transwell]], [[colony formation]], and [[flow cytometry]] assays. A [[mouse]] subcutaneous xenotransplanted [[tumor model]] was established. The expressions of miR-942-5p, KNG1, [[LINC01018]], and related genes were evaluated by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR), [[Western blot]], or [[immunohistochemistry]].

MiR-942-5p targeted KNG1, and LINC01018 sponged miR-942-5p. The high survival rate of patients was related to low miR-942-5p level. MiR-942-5p was highly expressed, whereas KNG1 was lowly expressed in glioma. MiR-942-5p was negatively correlated with KNG1. Silent LINC01018 or KNG1 and miR-942-5p mimic enhanced the migration, invasion, and proliferation of glioma cells, and regulated the expressions of metastasis-related and proliferation-related genes. LINC01018 knockdown and miR-942-5p mimic promoted glioma tumor growth in mice. The levels of miR-942-5p and KNG1 were decreased by LINC01018 knockdown, and LINC01018 expression was suppressed by miR-942-5p mimic. MiR-942-5p inhibitor, KNG1, and LINC01018 had the opposite effect to miR-942-5p mimic.

Conclusion: LINC01018/miR-942-5p/KNG1 pathway regulates the development of glioma cells in vitro and in vivo
((Xu J, Wang J, Zhao M, Li C, Hong S, Zhang J. LncRNA LINC01018/miR-942-5p/KNG1 axis regulates the malignant development of glioma in vitro and in vivo. CNS Neurosci Ther. 2022 Dec 22. doi: 10.1111/cns.14053. Epub ahead of print. PMID: 36550594.)).