A total of 50 pairs of [[glioma]] [[tissue]] samples and para-[[carcinoma]] tissue samples were collected. Human glioma cell line ([[U251]]) and normal human [[astrocyte]] (NHA) were cultured. The expression of [[RNA]] and [[protein]] was detected by quantitative [[real-time polymerase chain reaction]] (qRT-PCR) and [[Western blot]], respectively. [[Cell proliferation]] assay and transwell assay were used to detect the activities of proliferation and invasion. [[Luciferase]] reporter assays were carried out to determine the binding efficiency between forkhead box O1 ([[FOXO1]]) and miR-135a in U251 cells.

qRT-PCR results showed that miR-135a expression was significantly reduced while FOXO1 was up-regulated in glioma tissues. miR-135a overexpression in U251 cells could prominently inhibit proliferation and invasion according to the transwell assays. Moreover, FOXO1 was recognized as the target for miR-135a and may partially reverse the functions of miR-135a in U251 cells.

Shi et al., from the Yantaishan Hospital, Yantai, China, showed that miR-135a inhibits [[glioma cell]] proliferation and invasion by down-regulating the target gene FOXO1
((Shi HZ, Wang DN, Xu F, Teng JH, Wang YL. miR-135a inhibits glioma cell
proliferation and invasion by directly targeting FOXO1. Eur Rev Med Pharmacol
Sci. 2018 Jul;22(13):4215-4223. doi: 10.26355/eurrev_201807_15415. PubMed PMID:
30024610.
)).