=====Klingler method=====

The specimens were extracted during the first 12 h postmortem and placed in 10% [[formalin]] solution for 8 weeks. The brains were washed under running water and frozen to −15°C for 2 weeks
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During the 1930s, [[white matter tract]]s began to assume relevance for neurosurgery, especially after Cajal's work. In many reviews of white matter neurobiology, the seminal contributions of [[Josef Klingler]] (1888-1963) and their neurological applications have been overlooked
((Agrawal A, Kapfhammer JP, Kress A, Wichers H, Deep A, Feindel W, Sonntag VK,
Spetzler RF, Preul MC. Josef Klingler's models of white matter tracts: influences
on neuroanatomy, neurosurgery, and neuroimaging. Neurosurgery. 2011
Aug;69(2):238-52; discussion 252-4. doi: 10.1227/NEU.0b013e318214ab79. PubMed
PMID: 21368687.
)).

This freezing technique, first described by [[Josef Klingler]] and later revitalized by [[Uğur Türe]] et al. has already been reported to have a good correlation with [[diffusion tensor imaging]] ([[DTI]]) [[tractography]]
((Martino J, da Silva-Freitas R, Caballero H, Marco de Lucas
E, García-Porrero JA, Vázquez-Barquero A: Fiber dissection
and diffusion tensor imaging tractography study of the
temporoparietal fiber intersection area. Neurosurgery 72 (1
Suppl Operative):87–98, 2013)).