====== Göttingen minipig ======
 

The Göttingen mini[[pig]] is being used increasingly in biomedical research. The anatomical structure of the porcine peripheral nervous system has been extensively characterized, but no equivalent to the dermatome map, which is so valuable in human neurophysiological research, has been created. We characterized the medullar segmental skin and muscle innervations of the minipig hind body, using neurophysiological methodology. Six adult minipigs underwent unilateral laminectomy from L2 to S3, exposing the nerve roots. The skin of the hind part of the body was divided into 36 predefined fields, based on anatomical landmarks for consistent reproducibility. We recorded the evoked potential in each exposed nerve root L2-S3 for cutaneous stimulation of each skin field, mapping the sensory innervation of the entire hind body. We subsequently recorded the motor response in seven predefined muscles during sequential stimulation of the L2-S3 nerve roots. We obtained a clear sensory evoked potential in the nerve roots during stimulation of the skin fields, allowing us to map the sensory innervation of the minipig hind body. Neurophysiological data from skin stimulation and muscle recordings enabled us to map the sensory innervation of the Göttingen minipig hind body and provide information about muscular innervation. The skin fields were sensory innervated by more than one root. The muscles each had one dominant root with minor contribution from neighboring roots. This is consistent with experimental data from human studies
((Meier K, Qerama E, Ettrup KS, Glud AN, Alstrup AKO, Sørensen JCH. Segmental
innervation of the Göttingen minipig hind body. An electrophysiological study. J 
Anat. 2018 Jul 24. doi: 10.1111/joa.12865. [Epub ahead of print] PubMed PMID:
30040118.
)).
----
1: Steinmüller JB, Bjarkam CR, Orlowski D, Sørensen JCH, Glud AN. Anterograde
Tracing From the Göttingen Minipig Motor and Prefrontal Cortex Displays a
Topographic Subthalamic and Striatal Axonal Termination Pattern Comparable to
Previous Findings in Primates. Front Neural Circuits. 2021 Nov 26;15:716145.
doi: 10.3389/fncir.2021.716145. PMID: 34899195; PMCID: PMC8661455.

2: Fessler RG, Liu CY, McKenna S, Fessler RD, Lebkowski JS, Priest CA, Wirth ED.
Safety of direct injection of oligodendrocyte progenitor cells into the spinal
cord of uninjured Göttingen minipigs. J Neurosurg Spine. 2021 Jul 9:1-9. doi:
10.3171/2020.12.SPINE201853. Epub ahead of print. PMID: 34243160.

3: Tvilling L, West M, Glud AN, Zaer H, Sørensen JCH, Bjarkam CR, Orlowski D.
Anatomy and histology of the Göttingen minipig adenohypophysis with special
emphasis on the polypeptide hormones: GH, PRL, and ACTH. Brain Struct Funct.
2021 Sep;226(7):2375-2386. doi: 10.1007/s00429-021-02337-1. Epub 2021 Jul 7.
PMID: 34235563.

4: Zaer H, Deshmukh A, Orlowski D, Fan W, Prouvot PH, Glud AN, Jensen MB, Worm
ES, Lukacova S, Mikkelsen TW, Fitting LM, Adler JR Jr, Schneider MB, Jensen MS,
Fu Q, Go V, Morizio J, Sørensen JCH, Stroh A. An Intracortical Implantable
Brain-Computer Interface for Telemetric Real-Time Recording and Manipulation of
Neuronal Circuits for Closed-Loop Intervention. Front Hum Neurosci. 2021 Feb
3;15:618626. doi: 10.3389/fnhum.2021.618626. PMID: 33613212; PMCID: PMC7887289.

5: Bech J, Orlowski D, Glud AN, Dyrby TB, Sørensen JCH, Bjarkam CR. Ex vivo
diffusion-weighted MRI tractography of the Göttingen minipig limbic system.
Brain Struct Funct. 2020 Apr;225(3):1055-1071. doi: 10.1007/s00429-020-02058-x.
Epub 2020 Apr 3. PMID: 32246243.

6: Tora MS, Texakalidis P, Neill S, Wetzel J, Rindler RS, Hardcastle N,
Nagarajan PP, Krasnopeyev A, Roach C, James R, Bruce JN, Canoll P, Federici T,
Oshinski JN, Boulis NM. Lentiviral Vector Induced Modeling of High-Grade Spinal
Cord Glioma in Minipigs. Sci Rep. 2020 Mar 24;10(1):5291. doi:
10.1038/s41598-020-62167-9. PMID: 32210315; PMCID: PMC7093438.

7: Landau AM, Noer O, Alstrup AKO, Audrain H, Wegener G, Gjedde A, Doudet DJ,
Winterdahl M. Type of Anaesthetic Influences [<sup>11</sup>C]MDL100,907 Binding
to 5HT<sub>2A</sub> Receptors in Porcine Brain. Mol Imaging Biol. 2020
Aug;22(4):797-804. doi: 10.1007/s11307-020-01476-x. PMID: 31993926.

8: Ørstrup LH, Tvilling L, Orlowski D, Zaer H, Bjarkam CR, von Voss P, Andersen
PS, Christoffersen BØ, Hedemann Sørensen JC, Laursen T, Thygesen P, Lykkesfeldt
J, Glud AN. Towards a Göttingen minipig model of adult onset growth hormone
deficiency: evaluation of stereotactic electrocoagulation method. Heliyon. 2019
Nov 28;5(11):e02892. doi: 10.1016/j.heliyon.2019.e02892. PMID: 31844758; PMCID:
PMC6895662.

9: Winterdahl M, Noer O, Orlowski D, Schacht AC, Jakobsen S, Alstrup AKO, Gjedde
A, Landau AM. Sucrose intake lowers μ-opioid and dopamine D2/3 receptor
availability in porcine brain. Sci Rep. 2019 Nov 15;9(1):16918. doi:
10.1038/s41598-019-53430-9. PMID: 31729425; PMCID: PMC6858372.

10: Orlowski D, Glud AN, Palomero-Gallagher N, Sørensen JCH, Bjarkam CR.
Corrigendum to "Online histological atlas of the Göttingen minipig brain"
[Heliyon 5 (3) (March 2019) e01363]. Heliyon. 2019 Apr 30;5(4):e01530. doi:
10.1016/j.heliyon.2019.e01530. Erratum for: Heliyon. 2019 Mar 19;5(3):e01363.
Palomero-Galagher, Nicola [corrected to Palomero-Gallagher, Nicola]. PMID:
31183415; PMCID: PMC6497801.

11: Chun Y, Chen Y, Elsisy M, Sukinik J, Shridhar P, Park JK, Kim YB, Hong CK,
Chung J. In vitro and in vivo experiments of a novel intra-arterial
neurovascular decompressor for treating neurovascular compression syndromes: a
brief report. Neurol Res. 2019 Jul;41(7):665-670. doi:
10.1080/01616412.2019.1611009. Epub 2019 May 2. PMID: 31044660.

12: Orlowski D, Glud AN, Palomero-Gallagher N, Sørensen JCH, Bjarkam CR. Online
histological atlas of the Göttingen minipig brain. Heliyon. 2019 Mar
19;5(3):e01363. doi: 10.1016/j.heliyon.2019.e01363. Erratum in: Heliyon. 2019
Apr 30;5(4):e01530. PMID: 30949607; PMCID: PMC6429808.

13: Landau AM, Alstrup AKO, Noer O, Winterdahl M, Audrain H, Møller A, Videbech
P, Wegener G, Gjedde A, Doudet DJ. Electroconvulsive stimulation differentially
affects [<sup>11</sup>C]MDL100,907 binding to cortical and subcortical
5HT<sub>2A</sub> receptors in porcine brain. J Psychopharmacol. 2019
Jun;33(6):714-721. doi: 10.1177/0269881119836212. Epub 2019 Mar 19. PMID:
30887871.

14: Guest JD, Moore SW, Aimetti AA, Kutikov AB, Santamaria AJ, Hofstetter CP,
Ropper AE, Theodore N, Ulich TR, Layer RT. Internal decompression of the acutely
contused spinal cord: Differential effects of irrigation only versus
biodegradable scaffold implantation. Biomaterials. 2018 Dec;185:284-300. doi:
10.1016/j.biomaterials.2018.09.025. Epub 2018 Sep 19. PMID: 30265898.

15: Bech J, Glud AN, Sangill R, Petersen M, Frandsen J, Orlowski D, West MJ,
Pedersen M, Sørensen JCH, Dyrby TB, Bjarkam CR. The porcine corticospinal
decussation: A combined neuronal tracing and tractography study. Brain Res Bull.
2018 Sep;142:253-262. doi: 10.1016/j.brainresbull.2018.08.004. Epub 2018 Aug 4.
PMID: 30086351.



17: Lillethorup TP, Glud AN, Landeck N, Alstrup AKO, Jakobsen S, Vang K, Doudet
DJ, Brooks DJ, Kirik D, Hinz R, Sørensen JC, Landau AM. In vivo quantification
of glial activation in minipigs overexpressing human α-synuclein. Synapse. 2018
Dec;72(12):e22060. doi: 10.1002/syn.22060. Epub 2018 Aug 12. PMID: 30009467.

18: Christensen AB, Sørensen JCH, Ettrup KS, Orlowski D, Bjarkam CR. Pirouetting
pigs: A large non-primate animal model based on unilateral 6-hydroxydopamine
lesioning of the nigrostriatal pathway. Brain Res Bull. 2018 May;139:167-173.
doi: 10.1016/j.brainresbull.2018.02.010. Epub 2018 Feb 17. PMID: 29462643.

19: Lillethorup TP, Glud AN, Alstrup AKO, Mikkelsen TW, Nielsen EH, Zaer H,
Doudet DJ, Brooks DJ, Sørensen JCH, Orlowski D, Landau AM. Nigrostriatal
proteasome inhibition impairs dopamine neurotransmission and motor function in
minipigs. Exp Neurol. 2018 May;303:142-152. doi:
10.1016/j.expneurol.2018.02.005. Epub 2018 Feb 8. PMID: 29428213.

20: Lamanna JJ, Gutierrez J, Espinosa JR, Wagner J, Urquia LN, Moreton C, Victor
Hurtig C, Tora M, Kirk AD, Federici T, Boulis NM. Peripheral blood detection of
systemic graft-specific xeno-antibodies following transplantation of human
neural progenitor cells into the porcine spinal cord. J Clin Neurosci. 2018
Feb;48:173-180. doi: 10.1016/j.jocn.2017.10.033. Epub 2017 Oct 28. PMID:
29089163.

21: Glud AN, Bech J, Tvilling L, Zaer H, Orlowski D, Fitting LM, Ziedler D,
Geneser M, Sangill R, Alstrup AKO, Bjarkam CR, Sørensen JCH. A fiducial skull
marker for precise MRI-based stereotaxic surgery in large animal models. J
Neurosci Methods. 2017 Jun 15;285:45-48. doi: 10.1016/j.jneumeth.2017.04.017.
Epub 2017 May 1. PMID: 28472679.

22: Meidahl AC, Orlowski D, Sørensen JC, Bjarkam CR. The Retrograde Connections
and Anatomical Segregation of the Göttingen Minipig Nucleus Accumbens. Front
Neuroanat. 2016 Dec 5;10:117. doi: 10.3389/fnana.2016.00117. PMID: 27994542;
PMCID: PMC5136552.

23: Bjarkam CR, Glud AN, Orlowski D, Sørensen JCH, Palomero-Gallagher N. The
telencephalon of the Göttingen minipig, cytoarchitecture and cortical surface
anatomy. Brain Struct Funct. 2017 Jul;222(5):2093-2114. doi:
10.1007/s00429-016-1327-5. Epub 2016 Oct 24. PMID: 27778106.

24: Nielsen MS, Glud AN, Møller A, Mogensen P, Bender D, Sørensen JC, Doudet D,
Bjarkam CR. Continuous MPTP intoxication in the Göttingen minipig results in
chronic parkinsonian deficits. Acta Neurobiol Exp (Wars). 2016;76(3):199-211.
doi: 10.21307/ane-2017-020. PMID: 27685773.

25: Gutierrez J, Lamanna JJ, Grin N, Hurtig CV, Miller JH, Riley J, Urquia L,
Avalos P, Svendsen CN, Federici T, Boulis NM. Preclinical Validation of
Multilevel Intraparenchymal Stem Cell Therapy in the Porcine Spinal Cord.
Neurosurgery. 2015 Oct;77(4):604-12; discussion 612. doi:
10.1227/NEU.0000000000000882. PMID: 26134596.

26: Landau AM, Dyve S, Jakobsen S, Alstrup AK, Gjedde A, Doudet DJ. Acute Vagal
Nerve Stimulation Lowers α2 Adrenoceptor Availability: Possible Mechanism of
Therapeutic Action. Brain Stimul. 2015 Jul-Aug;8(4):702-7. doi:
10.1016/j.brs.2015.02.003. Epub 2015 Feb 13. PMID: 25758422.

27: Federici T, Hurtig CV, Burks KL, Riley JP, Krishna V, Miller BA, Sribnick
EA, Miller JH, Grin N, Lamanna JJ, Boulis NM. Surgical technique for spinal cord
delivery of therapies: demonstration of procedure in gottingen minipigs. J Vis
Exp. 2012 Dec 7;(70):e4371. doi: 10.3791/4371. PMID: 23242422; PMCID:
PMC3567164.

28: Ettrup KS, Sørensen JC, Rodell A, Alstrup AK, Bjarkam CR. Hypothalamic deep
brain stimulation influences autonomic and limbic circuitry involved in the
regulation of aggression and cardiocerebrovascular control in the Göttingen
minipig. Stereotact Funct Neurosurg. 2012;90(5):281-91. doi: 10.1159/000338087.
Epub 2012 Jul 11. PMID: 22797692.

29: Orakcioglu B, Beynon C, Kentar MM, Eymann R, Kiefer M, Sakowitz OW.
Intracranial pressure telemetry: first experience of an experimental in vivo
study using a new device. Acta Neurochir Suppl. 2012;114:105-10. doi:
10.1007/978-3-7091-0956-4_19. PMID: 22327673.

30: Sørensen JC, Nielsen MS, Rosendal F, Deding D, Ettrup KS, Jensen KN,
Jørgensen RL, Glud AN, Meier K, Fitting LM, Møller A, Alstrup AK, Ostergaard L,
Bjarkam CR. Development of neuromodulation treatments in a large animal model--
do neurosurgeons dream of electric pigs? Prog Brain Res. 2011;194:97-103. doi:
10.1016/B978-0-444-53815-4.00014-5. PMID: 21867797.

31: Ettrup KS, Glud AN, Orlowski D, Fitting LM, Meier K, Soerensen JC, Bjarkam
CR, Alstrup AK. Basic surgical techniques in the Göttingen minipig: intubation,
bladder catheterization, femoral vessel catheterization, and transcardial
perfusion. J Vis Exp. 2011 Jun 26;(52):2652. doi: 10.3791/2652. PMID: 21730947;
PMCID: PMC3197034.

32: Ettrup KS, Tornøe J, Sørensen JC, Bjarkam CR. A surgical device for
minimally invasive implantation of experimental deep brain stimulation leads in
large research animals. J Neurosci Methods. 2011 Aug 30;200(1):41-6. doi:
10.1016/j.jneumeth.2011.06.011. Epub 2011 Jun 22. PMID: 21723320.

33: Raore B, Federici T, Taub J, Wu MC, Riley J, Franz CK, Kliem MA, Snyder B,
Feldman EL, Johe K, Boulis NM. Cervical multilevel intraspinal stem cell
therapy: assessment of surgical risks in Gottingen minipigs. Spine (Phila Pa
1976). 2011 Feb 1;36(3):E164-71. doi: 10.1097/BRS.0b013e3181d77a47. PMID:
21099736.

34: Norgaard Glud A, Hedegaard C, Nielsen MS, Sørensen JC, Bendixen C, Jensen
PH, Larsen K, Bjarkam CR. Direct gene transfer in the Gottingen minipig CNS
using stereotaxic lentiviral microinjections. Acta Neurobiol Exp (Wars).
2010;70(3):308-15. PMID: 20871651.

35: Rosendal F, Chakravarty MM, Sunde N, Rodell A, Jonsdottir KY, Pedersen M,
Bjarkam C, Sørensen JC. Defining the intercommissural plane and stereotactic
coordinates for the Basal Ganglia in the Göttingen minipig brain. Stereotact
Funct Neurosurg. 2010;88(3):138-46. doi: 10.1159/000303526. Epub 2010 Apr 1.
PMID: 20357521.

36: Bjarkam CR, Glud AN, Margolin L, Reinhart K, Franklin R, Deding D, Ettrup
KS, Fitting LM, Nielsen MS, Sørensen JC, Cunningham MG. Safety and function of a
new clinical intracerebral microinjection instrument for stem cells and
therapeutics examined in the Göttingen minipig. Stereotact Funct Neurosurg.
2010;88(1):56-63. doi: 10.1159/000268743. Epub 2009 Dec 24. PMID: 20051711.

37: Ettrup KS, Sørensen JC, Bjarkam CR. The anatomy of the Göttingen minipig
hypothalamus. J Chem Neuroanat. 2010 May;39(3):151-65. doi:
10.1016/j.jchemneu.2009.12.004. Epub 2009 Dec 28. PMID: 20043984.

38: Rosendal F, Frandsen J, Chakravarty MM, Bjarkam CR, Pedersen M, Sangill R,
Sørensen JC. New surgical technique reduces the susceptibility artefact at air-
tissue interfaces on in vivo cerebral MRI in the Göttingen minipig. Brain Res
Bull. 2009 Dec 16;80(6):403-7. doi: 10.1016/j.brainresbull.2009.08.012. Epub
2009 Aug 25. PMID: 19712728.

39: Jensen KN, Deding D, Sørensen JC, Bjarkam CR. Long-term implantation of deep
brain stimulation electrodes in the pontine micturition centre of the Göttingen
minipig. Acta Neurochir (Wien). 2009 Jul;151(7):785-94; discussion 794. doi:
10.1007/s00701-009-0334-1. Epub 2009 Apr 30. PMID: 19404572.

40: Rosendal F, Pedersen M, Sangill R, Stødkilde-Jørgensen H, Nielsen MS,
Bjarkam CR, Sunde N, Sørensen JC. MRI protocol for in vivo visualization of the
Göttingen minipig brain improves targeting in experimental functional
neurosurgery. Brain Res Bull. 2009 Apr 6;79(1):41-5. doi:
10.1016/j.brainresbull.2009.01.002. Epub 2009 Jan 29. PMID: 19185604.

41: Bjarkam CR, Jorgensen RL, Jensen KN, Sunde NA, Sørensen JC. Deep brain
stimulation electrode anchoring using BioGlue((R)), a protective electrode
covering, and a titanium microplate. J Neurosci Methods. 2008 Feb
15;168(1):151-5. doi: 10.1016/j.jneumeth.2007.09.008. Epub 2007 Sep 18. PMID:
17953993.

42: Andersen F, Watanabe H, Bjarkam C, Danielsen EH, Cumming P; DaNeX Study
Group. Pig brain stereotaxic standard space: mapping of cerebral blood flow
normative values and effect of MPTP-lesioning. Brain Res Bull. 2005 Jul
15;66(1):17-29. doi: 10.1016/j.brainresbull.2005.02.033. Epub 2005 Apr 26. PMID:
15925140.

43: Bjarkam CR, Cancian G, Larsen M, Rosendahl F, Ettrup KS, Zeidler D,
Blankholm AD, Østergaard L, Sunde N, Sørensen JC. A MRI-compatible stereotaxic
localizer box enables high-precision stereotaxic procedures in pigs. J Neurosci
Methods. 2004 Oct 30;139(2):293-8. doi: 10.1016/j.jneumeth.2004.05.004. PMID:
15488243.

44: Andersen T, Christensen FB, Laursen M, Lund-Olesen L, Gelineck J, Bünger C.
In vitro osteoblast proliferation as a predictor for spinal fusion mass. Spine
J. 2003 Jul-Aug;3(4):285-8. doi: 10.1016/s1529-9430(03)00030-5. PMID: 14589188.

45: Dall AM, Danielsen EH, Sørensen JC, Andersen F, Møller A, Zimmer J, Gjedde
AH, Cumming P; Danish Neuronal Xenografting Group. Quantitative
[18F]fluorodopa/PET and histology of fetal mesencephalic dopaminergic grafts to
the striatum of MPTP-poisoned minipigs. Cell Transplant. 2002;11(8):733-46.
PMID: 12588105.

46: Verheggen R, Merten HA. Correction of skull defects using hydroxyapatite
cement (HAC)--evidence derived from animal experiments and clinical experience.
Acta Neurochir (Wien). 2001 Sep;143(9):919-26. doi: 10.1007/s007010170022. PMID:
11685624.

47: Wiltfang J, Merten HA, Schultze-Mosgau S, Schrell U, Wénzel D, Kessler P.
Biodegradable miniplates (LactoSorb): long-term results in infant minipigs and
clinical results. J Craniofac Surg. 2000 May;11(3):239-43; discussion 244-5.
doi: 10.1097/00001665-200011030-00006. PMID: 11314302.

48: Danielsen EH, Cumming P, Andersen F, Bender D, Brevig T, Falborg L, Gee A,
Gillings NM, Hansen SB, Hermansen F, Johansen J, Johansen TE, Dahl-Jørgensen A,
Jørgensen HA, Meyer M, Munk O, Pedersen EB, Poulsen PH, Rodell AB, Sakoh M,
Simonsen CZ, Smith DF, Sørensen JC, Ostergård L, Zimmer J, Gjedde A, Møller A.
The DaNeX study of embryonic mesencephalic, dopaminergic tissue grafted to a
minipig model of Parkinson's disease: preliminary findings of effect of MPTP
poisoning on striatal dopaminergic markers. Cell Transplant. 2000 Mar-
Apr;9(2):247-59. doi: 10.1177/096368970000900210. PMID: 10811397.

49: Wiltfang J, Merten HA, Becker HJ, Luhr HG. The resorbable miniplate system
Lactosorb in a growing cranio-osteoplasty animal model. J Craniomaxillofac Surg.
1999 Aug;27(4):207-10. doi: 10.1016/s1010-5182(99)80030-4. PMID: 10626252.

50: Becker HJ, Wiltfang J, Merten HA, Luhr HG. Biodegradierbare Miniplatten
(Lactosorb) bei Kranioosteoplastik--experimentelle Ergebnisse am schnell
wachsenden, juvenilen Miniaturschwein [Biodegradable miniplates (lactosorb) in
cranio-osteoplasty--experimental results with the rapidly maturing, juvenile
minipig]. Mund Kiefer Gesichtschir. 1999 Sep;3(5):275-8. German. doi:
10.1007/s100060050150. PMID: 10540829.

51: Luhr HG, Merten HA, Becker HJ. Bedeutung des Periosts bei profil- und
konturverbessernden Osteotomien am wachsenden Schädel. Tierexperimentelle
Untersuchungen am juvenilen Minipig [Significance of periosteum in profile and
contour improving osteotomies of the growing skull. Animal experiment studies
with the juvenile minipig]. Mund Kiefer Gesichtschir. 1997 May;1 Suppl
1:S149-52. German. PMID: 9424369.

52: Hönig JF, Merten HA, Luhr HG. Passive and active intracranial translocation
of osteosynthesis plates in adolescent minipigs. J Craniofac Surg. 1995
Jul;6(4):292-8; discussion 299-300. doi: 10.1097/00001665-199507000-00006. PMID:
9020704.

53: Speakman MJ, Brading AF, Gilpin CJ, Dixon JS, Gilpin SA, Gosling JA. Bladder
outflow obstruction--a cause of denervation supersensitivity. J Urol. 1987
Dec;138(6):1461-6. doi: 10.1016/s0022-5347(17)43675-5. PMID: 3682077.