Aggressive [[metastasis]] of [[tumor cell]]s assumed a constructive role in strengthening the [[chemoresistance]] of tumors, so this investigation was intended to elucidate if lncRNA CCAT2 sponging downstream [[miR 424]] regulated chemotolerance of glioma cells by boosting metastasis of glioma cells. One hundred and twenty-eight pairs of [[glioma]] tissues and corresponding adjacent [[tissue]]s were resected from glioma patients during their operation, and we also purchased a series of glioma cell lines, including [[U251]], [[U87]], [[A172]] and [[SHG44]]. Furthermore, pcDNA3.1-CCAT2, si-CCAT2, miR-424 mimic and miR-424 inhibitor were transfected into SHG44 and U251 [[cell line]]s, so as to evaluate impacts of CCAT2 and miR-424 on chemosensitivity of the glioma cells. Besides, proliferation, invasion, and metastasis of the cells were determined through the implementation of [[colony formation assay]], [[transwell assay]] and [[scratch assay]]. Glioma tissues and cells were monitored with higher CCAT2 expression and lower miR-424 expression than adjacent normal tissues and NHA cell line (P<0.05). Among the glioma cell lines, the SHG44 cell line showed the strongest resistance against teniposide, temozolomide and cisplatin (P<0.05), whereas the U251 cell line was more sensitive to teniposide, temozolomide, vincristine and cisplatin than any other cell line (P<0.05). Besides, pcDNA3.1-CCAT2 and miR-424 inhibitor could enhance tolerance of glioma cell lines against drugs (P<0.05). Moreover, in-vitro transfection of si-CCAT2 and miR-424 mimic could significantly retard proliferation, invasion and migration of SHG44 and U251 cells (P<0.05), and CCAT2 was found to negatively regulate miR-424 expression by sponging it (P<0.05). In addition, CHK1 was deemed as the molecule targeted by upstream miR-424, and its overexpression can changeover the effects of miR-424 mimic on proliferation and metastasis of SHG44 and U251 cells. lncRNA CCAT2/miR-424/Chk1 axis might serve as a promising target for improving chemotherapeutic efficacies in glioma treatment ((Ding J, Zhang L, Chen S, Cao H, Xu C, Wang X. lncRNA CCAT2 Enhanced Resistance of Glioma Cells Against Chemodrugs by Disturbing the Normal Function of miR-424. Onco Targets Ther. 2020 Feb 17;13:1431-1445. doi: 10.2147/OTT.S227831. eCollection 2020. PubMed PMID: 32110042; PubMed Central PMCID: PMC7034969. )).