yin_yang_1 [Neurosurgery Wiki]

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
Yin Yang‑1 (YY1), a [[transcription factor]] that is part of the [[polycomb group protein]] (PcG) family, is a direct target of [[miR218]] in human glioma cells. It was demonstrated that YY1 promoted glioma cell proliferation and miR‑218 could inhibit glioma cell proliferation by targeting YY1, and indirectly reduced the degradation of p53. Together the results indicate that miR‑218 functions as a tumor suppressor in human glioma and suggest that overexpression of miR‑218 may be a potential strategy for the treatment of human glioma in the future
((Gao Y, Sun L, Wu Z, Xuan C, Zhang J, You Y, Chen X. miR‑218 inhibits the
proliferation of human glioma cells through downregulation of Yin Yang 1. Mol Med
Rep. 2017 Nov 15. doi: 10.3892/mmr.2017.8063. [Epub ahead of print] PubMed PMID: 
29138857.
))
----
Although transcription factor Yin Yang 1 (YY1) has been reported to have a dual activator/repressor role during the regulation of interferon beta (IFN-β) promoter activity, the roles of YY1 in the regulation of upstream signaling pathways leading to IFN-1 induction and IFN-1 signaling during viral infection remain to be elucidated.
METHODS:

The roles of YY1 in IFN-1 production and IFN-1 signaling were investigated using immunoblotting, real-time PCR, small interfering RNA (siRNA)-mediated YY1 knockdown, YY1 overexpression by transient transfection, and co-immunoprecipitation, using mouse cells.
RESULTS:

YY1 was shown to interact with STAT1 in the absence of viral infection. Following viral infection, YY1 protein expression levels were decreased. YY1 knockdown led to a considerable downregulation of phosphorylated (p) TBK1 and pIRF3 expressions, while YY1 overexpression significantly upregulated pTBK1 and pIRF3 expression levels and promoted virus-induced IFN-β production. Additionally, YY1 knockdown led to a significant upregulation of pSTAT1, pSTAT2 and antiviral interferon-stimulated genes, and inhibited viral replication.
CONCLUSION:

We demonstrated here that YY1 interacts with STAT1 and dynamically regulates the induction of IFN-1 production and activation of IFN-1 signaling in different stages during viral infection
((Zan J, Zhang H, Gu AP, Zhong KL, Lu MY, Bai XX, Zhang JY, Cai J. Yin Yang 1
Dynamically Regulates Antiviral Innate Immune Responses During Viral Infection.
Cell Physiol Biochem. 2017 Nov 20;44(2):607-617. doi: 10.1159/000485116. [Epub
ahead of print] PubMed PMID: 29161701.)).