VSV-G Fusion [Neurosurgery Wiki]

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===== VSV-G Fusion =====

**VSV-G fusion** refers to the use of the vesicular stomatitis virus glycoprotein G (VSV-G) to enhance the fusion of engineered delivery systems—such as extracellular vesicles (EVs)—with the plasma membrane of target cells.

==== Key Features ====
  * Envelope glycoprotein from Vesicular Stomatitis Virus
  * Enables pH-dependent membrane fusion in endosomes
  * Broad host cell range (non-specific tropism)
  * Used to pseudotype viral and EV-based delivery vectors

==== In EV Engineering ====
VSV-G is incorporated into EV membranes to:
  * Promote efficient fusion with recipient cells
  * Improve cytosolic delivery of loaded therapeutic cargo (e.g., Cas9, Cre)
  * Enhance in vivo delivery efficiency

==== Limitations ====
  * Immunogenic in clinical settings
  * Lacks tissue specificity