Primary cell culture [Neurosurgery Wiki]

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====== Primary cell culture ======

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A high-density 3D primary cell culture model for short-term testing from resected [[glioblastoma]] [[tissue]] that is set up on the day of surgery, established within 7 days, and viable for at least 3 weeks. High-density 3D [[culture]]s contain [[tumor]] and host cells, including [[microglia]], and retain key histopathological characteristics of their parent tumors, including proliferative activity, expression of the marker [[GFAP]], and presence of giant cells. This provides proof of concept that 3D primary cultures may be useful to model tumor heterogeneity. High-density 3D cultures can be used to test chemotherapy response within a 2-3-week timeframe and are predictive of patient response to Temozolomide therapy. Thus, primary high-density 3D cultures could be a useful tool for brain cancer research and the prediction of therapeutic [[resistance]]
((J B, M Z, C O, F S, R S, H B, Fa S, Wp G. A high-density 3-dimensional culture model of human glioblastoma for rapid screening of therapeutic resistance. Biochem Pharmacol. 2023 Jan 9:115410. doi: 10.1016/j.bcp.2023.115410. Epub ahead of print. PMID: 36632958.))