miR 29a [Neurosurgery Wiki]

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====== miR 29a ======

[[Grb2]]-associated binding 1 ([[Gab1]]) expression and microRNA-29a-3p ("miR-29a-3p") expression in human [[glioma cell]]s and [[tissue]]s were tested by [[Western blot]]ting [[assay]] and q[[RT-PCR]] assay. [[shRNA]]/[[siRNA]] strategy was applied to silence Gab1 in human glioma cells. miR-29a or anti-sense miR-29a construct was transfected to human glioma cells. [[Cell proliferation]] was tested by [[BrdU]] [[ELISA]] assay and [[cell counting]] assay.

Shao et al., from the 
Department of Neurosurgery, the Third Affiliated Hospital of Soochow University, [[Changzhou]], [[China]] show that expression of Gab1 was significantly elevated in human [[glioma]] tissues and [[cell]]s, which correlated with [[downregulation]] of its putative [[microRNA]]: miR-29a-3p. In [[A172]] glioma cells and primary human glioma cells, Gab1 shRNA/siRNA inhibited [[Akt]]-[[Erk]] activation and cell proliferation. Forced-expression of miR-29a-3p downregulated Gab1, inhibiting glioma cell proliferation, whereas miR-29a-3p was in-effective on cell proliferation in Gab1-silenced A172 cells. Furthermore, introduction of a 3'-untranslated region (3'-UTR) mutant Gab1 (UTR-G160A) blocked miR-29a-3p-induced inhibition on Akt signaling and A172 cell proliferation.

miR-29a-3p downregulation leads to Gab1 upregulation to promote glioma cell proliferation
((Shao NY, Wang DX, Wang Y, Li Y, Zhang ZQ, Jiang Q, Luo W, Cao C.
MicroRNA-29a-3p Downregulation Causes Gab1 Upregulation to Promote Glioma Cell
Proliferation. Cell Physiol Biochem. 2018 Jul 17;48(2):450-460. doi:
10.1159/000491776. [Epub ahead of print] PubMed PMID: 30016785.
)).