miR 200 [Neurosurgery Wiki]

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====== miR 200 ======

[[miR 16]], [[miR 143]], and [[miR 200]] showed statically significant higher [[expression]] among cases with cerebral aneurysms in comparison to controls. Thus, these preliminary [[result]]s of miRNAs [[biomarker]]s are promising future tool to be used for aneurysmal [[screening]]
((Al-Jehani HM, Turkistani AN, Alrayes MM, AlHamid MA, AlShamikh AS, Al Sadah ZM, Alabbas FM, Cyrus C, Al-Ali AK. The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms. Neurosciences (Riyadh). 2023 Oct;28(4):250-257. doi: 10.17712/nsj.2023.4.20230028. PMID: 37844954.))

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Spinal cord injury (SCI) is a common disease with high incidence, disability rate, and treatment cost. [[microRNA]] (miR)-200a is reported to inhibit [[Keap1]] to activate [[Nrf2]] signaling. This study aimed to explore the effects of lentivirus-mediated miR-200a gene-modified bone marrow mesenchymal stem cells (BMSCs) transplantation on the repair of SCI in a rat model. BMSCs were isolated from the bone marrow of Sprague-Dawley rats. miR-200a targeting to Keap1 was identified by luciferase-reporter gene assay. The expressions of Keap1, Nrf2, NQO-1, HO-1 and GCLC were detected by Western blotting in SCI rats. The locomotor capacity of the rats was evaluated using the Basso, Beattie and Bresnahan scale. The levels of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) and catalase (CAT) were measured. miR-200a inhibited Keap-1 3' UTR activity in BMSCs. Transplantation of BMSCs with overexpression of miR-200a or si-Keap1increased locomotor function recovery of rats after SCI, while decreased MDA level, increased SOD, CAT activities and Nrf2 expression together with its downstream HO-1, NQO1, GCLC protein expressions in SCI rat. These results indicated that overexpressed miR-200a in BMSCs promoted SCI repair, which may be through regulating anti-oxidative signaling pathway
((Wang X, Ye L, Zhang K, Gao L, Xiao J, Zhang Y. Upregulation of microRNA-200a
in bone marrow mesenchymal stem cells enhances the repair of spinal cord injury
in rats by reducing oxidative stress and regulating Keap1/Nrf2 pathway. Artif
Organs. 2020 Jan 29. doi: 10.1111/aor.13656. [Epub ahead of print] PubMed PMID:
31995644.
)).