Circular PVT1 [Neurosurgery Wiki]

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====== Circular PVT1 ======

Chi et al. investigated the function of [[circPVT1]] on [[GBM]].

[[CCK-8]] and [[flow cytometry]] were utilised to estimate viability and [[apoptosis]] in both [[cell]]s. [[qRT-PCR]] was performed to determine [[circPVT1]] and [[miR-199a-5p]] expression. [[Western blot]] was conducted to determine [[apoptosis]], [[migration]] and [[EMT]]-related proteins levels when silencing [[circPVT1]]. Subsequently, these parameters were re-tested after up-regulating miR-199a-5p.

CircPVT1 was highly expressed in GBM tissues. Silencing circPVT1 raised two cells apoptosis and reduced viability and migration capacity. Moreover, EGF-induced EMT was repressed by silencing circPVT1. In addition, miR-199a-5p expression was elevated when silencing circPVT1. And silencing circPVT1 exerted above changes via up-regulating miR-199a-5p. Finally, silencing circPVT1 repressed YAP1 and [[PI3K/AKT]] pathways via up-regulating miR-199a-5p.

This data suggested that silencing circPVT1 inhibited viability, migration, EGF-induced EMT and promoted apoptosis as well as repressed YAP1 and PI3K/AKT pathways by up-regulating miR-199a-5p.HIGHLIGHTSCircPVT1 expression is highly expressed in GBM tissues;Si-circPVT1 represses migration and promoted apoptosis in U539 and U251 cells;Si-circPVT1 represses migration and promoted apoptosis when elevating miR-199a-5p;Si-circPVT1 represses EGF-induced EMT when increasing miR-199a-5p;Si-circPVT1 suppresses [[YAP1]] and PI3K/AKT pathways by up-regulating miR-199-5p
((Chi G, Yang F, Xu D, Liu W. Silencing hsa_circ_PVT1 (circPVT1) suppresses the 
growth and metastasis of glioblastoma multiforme cells by up-regulation of
miR-199a-5p. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):188-196. doi:
10.1080/21691401.2019.1699825. PubMed PMID: 31865777.
)).