CircNDC80 [Neurosurgery Wiki]

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====== CircNDC80 ======
[[Circular RNA]]s (circRNAs) have been shown to be essential for the emergence and growth of different[[ cancer]]s. However, further research is required to validate the function of circRNA in [[glioblastoma]] (GBM).

[[CircNDC80]] expression in both normal [[brain tissue]]s (NBTs) and [[glioma]] tissues was determined using real-time [[PCR]]. The impact of circNDC80 on GBM [[cell proliferation,]] [[migration]], and [[invasion]] was then confirmed by [[CCK-8]], [[colony formation]], [[EdU]] incorporation, [[Transwell]], and [[wound-healing assay]]s. To determine how circNDC80 affects the capacity of glioma [[stem cell]]s (GSCs) to maintain their stemness and self-renewal, a [[CellTiter-Glo]] [[assay]], [[clonogenic assay]] and extreme [[limiting dilution assay]] were utilized. To ascertain the impact of circNDC80 in vivo, [[intracranial]] [[xenograft]] [[model]]s were established.

When compared to NBT, glioblastoma tissue had a higher level of circNDC80 expression. In functional assays, circNDC80 promoted glioblastoma cell proliferation, migration, and invasion, while sustaining the stemness and fostering the self-renewal of glioma stem cells. In addition, a dual luciferase reporter assay and circRIP were used to verify that circNDC80 simultaneously affects the expression of ECE1 mRNA by sponging miR-139-5p, and a rescue experiment was used to verify the above results further.

circNDC80 is an [[oncogenic transcription factor]] that promotes glioblastoma through the miR-139-5p/ECE1 pathway. This implies that circNDC80 may be employed as a novel therapeutic target and a possible predictive [[biomarker]]
((Wang Y, Wang B, Zhou F, Lv K, Xu X, Cao W. CircNDC80 promotes glioblastoma multiforme tumorigenesis via the miR-139-5p/ECE1 pathway. J Transl Med. 2023 Jan 12;21(1):22. doi: 10.1186/s12967-022-03852-3. PMID: 36635757.)).