Show pageBacklinksCite current pageExport to PDFBack to top This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong. ====== Cancer cell line ====== {{rss>https://pubmed.ncbi.nlm.nih.gov/rss/search/1-u-VnOuHrrMiM7m7o_VsILjbxyeem4PYRMs5b_stoRt5_nEPX/?limit=15&utm_campaign=pubmed-2&fc=20250402154952}} A cancer [[cell line]] is a [[population]] of [[cancer cell]]s that has been cultured in the [[laboratory]] and can continuously grow and divide. These cells originate from a tumor [[sample]] taken from a patient and are modified to survive and proliferate indefinitely under lab conditions. They're a crucial tool in [[cancer research]], drug development, and understanding cancer biology. 🔬 Characteristics: Immortalized: Unlike normal cells, they don't undergo senescence (aging). Genetically unstable: They may accumulate mutations over time. Homogeneous (sort of): All cells in the line are genetically similar, but some heterogeneity can still emerge. Adapted to 2D culture: Most are grown in monolayers, although 3D models (e.g., organoids) are increasingly used. 🧪 Common Cancer Cell Lines: Cell Line Cancer Type Origin HeLa Cervical cancer Henrietta Lacks (1951) MCF-7 Breast cancer Human A549 Lung carcinoma Human U87 Glioblastoma Human PC-3 Prostate cancer Human ⚠️ Limitations: May not fully represent real tumors (e.g., tumor microenvironment is absent). Long-term passaging can change their properties. Some are contaminated or misidentified (HeLa famously contaminated many lines). 🧰 Applications: Drug screening Gene editing and CRISPR studies Tumor biology research Biomarker discovery ---- An immortalized [[cell]] line is a population of cells from a multicellular organism that would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods in vitro. The mutations required for immortality can occur naturally or be intentionally induced for experimental purposes. Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. Immortalised cell lines have also found uses in biotechnology. https://www.atcc.org/~/media/PDFs/Brain_Tumor_Cell_Lines.ashx AM-38 GaMg D-54Mg CCF-STTG1 SW 1088 SW 1783 CHLA-02-ATRT [[A172]] U-138 MG LN-18 LN-229 [[U87]] MG U-118 MG T98G Hs 683 CHLA-01-MED CHP-212 H4 D341 Med Daoy PFSK-1 DBTRG-05MG M059K M059J IMR-32 BC3H1 bEnd.3 Neuro-2a NB41A3 N1E-115 [[C6]] C6/LacZ 9L/lacZ C6/lacZ7 F98EGFR F98npEGFRvIII F98 RG2 [[9L gliosarcoma]] [[U251]], cancer_cell_line.txt Last modified: 2025/04/02 19:50by 127.0.0.1