Show pageBacklinksCite current pageExport to PDFBack to top This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong. ====== Artificial cerebrospinal fluid ====== Artificial [[cerebrospinal fluid]] (aCSF) is a [[buffer solution]] that is used experimentally to immerse isolated brains, brain slices, or exposed brain regions to supply oxygen, maintain osmolarity, and to buffer pH at biological levels. ACSF is commonly used for electrophysiology experiments to maintain the neurons that are being studied. ===== Composition ===== One protocol for electrophysiology recording suggests the following composition for aCSF, with the pH and oxygen level stabilized by bubbling with carbogen (95% O2 and 5% CO2): 127 mM NaCl 1.0 mM KCl 1.2 mM KH2PO4 26 mM NaHCO3 10 mM D-glucose 2.4 mM CaCl2 1.3 mM MgCl2 artificial_cerebrospinal_fluid.txt Last modified: 2024/06/07 02:57by 127.0.0.1