4_mirna_signature [Neurosurgery Wiki]

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Although O(6)-methylguanine DNA methyltransferase (MGMT) promoter methylation status is an important marker for glioblastoma multiforme (Glioblastoma), there is considerable variability in the clinical outcome of patients with similar methylation profles.
OBJECTIVE:
We examined whether a MicroRNA (MicroRNA) signature can be identified for predicting clinical outcomes and helping in treatment decisions.
METHODS:
The differentially expressed MicroRNAs were evaluated in 6 pairs of short- (⩽ 450 days) and long-term survivors (> 450 days) by using microarray. real-time quantitative PCR (qRT-PCR) was applied to further verify screened MicroRNAs with a greater number of samples (n= 48). Meanwhile, functional interpretation of MicroRNA profile was carried out based on MicroRNA-target databases. In addition, MGMT promoter methylation status was tested by means of pyrosequencing (PSQ) testing.
RESULTS:
Six MicroRNAs were upregulated in the long-term survival group (fold change ⩾ 2.0, P< 0.05). The further verification by qRT-PCR indicated that the increase in let-7g-5p, miR-139-5p, miR-17-5p and miR-9-3p level in long-term survivors was statistically significant. Kaplan-Meier survival analysis showed that high expression of a prognostic 4-MicroRNA signature was significantly associated with good patient survival (p= 0.0012). The signature regulated signaling pathways including Calcium, MAPK, ErbB, mTOR and cell cycle involved in carcinogenesis from glial progenitor cell to primary Glioblastoma.
CONCLUSIONS:
The 4-MicroRNA signature was identified as an independent prognostic biomarker that identified patients who have a favorable outcome
((Yuan GQ, Wei NL, Wang XQ, Zhang YN, Mu LY, Zhou WN, Pan YW. A 4-MicroRNAs
signature predicts survival in glioblastoma multiforme patients. Cancer Biomark. 
2017 Aug 25. doi: 10.3233/CBM-170205. [Epub ahead of print] PubMed PMID:
28869437.
)).